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mouse anti human ccl22  (R&D Systems)


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    R&D Systems mouse anti human ccl22
    FIGURE 1 Circulating CCL17 and <t>CCL22</t> chemokine levels in morbidly obese patients and age-matched controls. (A) CCL17 and (B) CCL22 levels were measured in plasma samples from obese patients (n = 60) and controls (n = 20). Scatter dot plots showing median with interquartile range. Comparison between groups were made by Mann Whitney test. Spearman test shows a positive correlation between CCL17 and CCL22 with HOMA- IR Index (C, D) and BMI (E, F) (n = 20 control subjects and n = 60 morbidly obese patients).
    Mouse Anti Human Ccl22, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mouse anti human ccl22 - by Bioz Stars, 2026-03
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    1) Product Images from "CCL17 and CCL22 chemokines are upregulated in human obesity and play a role in vascular dysfunction."

    Article Title: CCL17 and CCL22 chemokines are upregulated in human obesity and play a role in vascular dysfunction.

    Journal: Frontiers in endocrinology

    doi: 10.3389/fendo.2023.1154158

    FIGURE 1 Circulating CCL17 and CCL22 chemokine levels in morbidly obese patients and age-matched controls. (A) CCL17 and (B) CCL22 levels were measured in plasma samples from obese patients (n = 60) and controls (n = 20). Scatter dot plots showing median with interquartile range. Comparison between groups were made by Mann Whitney test. Spearman test shows a positive correlation between CCL17 and CCL22 with HOMA- IR Index (C, D) and BMI (E, F) (n = 20 control subjects and n = 60 morbidly obese patients).
    Figure Legend Snippet: FIGURE 1 Circulating CCL17 and CCL22 chemokine levels in morbidly obese patients and age-matched controls. (A) CCL17 and (B) CCL22 levels were measured in plasma samples from obese patients (n = 60) and controls (n = 20). Scatter dot plots showing median with interquartile range. Comparison between groups were made by Mann Whitney test. Spearman test shows a positive correlation between CCL17 and CCL22 with HOMA- IR Index (C, D) and BMI (E, F) (n = 20 control subjects and n = 60 morbidly obese patients).

    Techniques Used: Clinical Proteomics, Comparison, MANN-WHITNEY, Control

    FIGURE 2 Expression of CCL17 and CCL22 is increased in VCAT from morbidly obese patients. Relative quantification of mRNA levels for (A) CCL17 and (B) CCL22. Comparisons between groups were made by Wilcoxon matched-pair signed-rank test. Values are expressed as mean ± SEM (n = 33). (C) CCL17 and (D) CCL22 chemokine release into conditioned media was determined after 48 h of SCAT and VCAT explant culture. Chemokine secretion is expressed as pg/ml in the supernatant. Values are expressed as mean ± SEM (n = 22). Comparison between groups were made by Mann Whitney test. (E) Immunofluorescence representative images showing colocalization of CCL17 with CD3 (lymphocytes), CD31 (endothelial cells) and Mac-3 (macrophages); or CCL22 with CD3, CD31, Mac-3 in VCAT. Immunoreactivity was visualized using Alexa Fluor 594 (CCL17 and CCL22, red) and Alexa Fluor 488 (CD31, CD3, Mac-3, green) secondary antibodies. Nuclei were stained with Hoechst (blue). Scale bar, 20 mm. Nuclei were stained with Hoechst (blue).
    Figure Legend Snippet: FIGURE 2 Expression of CCL17 and CCL22 is increased in VCAT from morbidly obese patients. Relative quantification of mRNA levels for (A) CCL17 and (B) CCL22. Comparisons between groups were made by Wilcoxon matched-pair signed-rank test. Values are expressed as mean ± SEM (n = 33). (C) CCL17 and (D) CCL22 chemokine release into conditioned media was determined after 48 h of SCAT and VCAT explant culture. Chemokine secretion is expressed as pg/ml in the supernatant. Values are expressed as mean ± SEM (n = 22). Comparison between groups were made by Mann Whitney test. (E) Immunofluorescence representative images showing colocalization of CCL17 with CD3 (lymphocytes), CD31 (endothelial cells) and Mac-3 (macrophages); or CCL22 with CD3, CD31, Mac-3 in VCAT. Immunoreactivity was visualized using Alexa Fluor 594 (CCL17 and CCL22, red) and Alexa Fluor 488 (CD31, CD3, Mac-3, green) secondary antibodies. Nuclei were stained with Hoechst (blue). Scale bar, 20 mm. Nuclei were stained with Hoechst (blue).

    Techniques Used: Expressing, Comparison, MANN-WHITNEY, Staining



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    FIGURE 1 Circulating CCL17 and <t>CCL22</t> chemokine levels in morbidly obese patients and age-matched controls. (A) CCL17 and (B) CCL22 levels were measured in plasma samples from obese patients (n = 60) and controls (n = 20). Scatter dot plots showing median with interquartile range. Comparison between groups were made by Mann Whitney test. Spearman test shows a positive correlation between CCL17 and CCL22 with HOMA- IR Index (C, D) and BMI (E, F) (n = 20 control subjects and n = 60 morbidly obese patients).
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    Image Search Results


    FIGURE 1 Circulating CCL17 and CCL22 chemokine levels in morbidly obese patients and age-matched controls. (A) CCL17 and (B) CCL22 levels were measured in plasma samples from obese patients (n = 60) and controls (n = 20). Scatter dot plots showing median with interquartile range. Comparison between groups were made by Mann Whitney test. Spearman test shows a positive correlation between CCL17 and CCL22 with HOMA- IR Index (C, D) and BMI (E, F) (n = 20 control subjects and n = 60 morbidly obese patients).

    Journal: Frontiers in endocrinology

    Article Title: CCL17 and CCL22 chemokines are upregulated in human obesity and play a role in vascular dysfunction.

    doi: 10.3389/fendo.2023.1154158

    Figure Lengend Snippet: FIGURE 1 Circulating CCL17 and CCL22 chemokine levels in morbidly obese patients and age-matched controls. (A) CCL17 and (B) CCL22 levels were measured in plasma samples from obese patients (n = 60) and controls (n = 20). Scatter dot plots showing median with interquartile range. Comparison between groups were made by Mann Whitney test. Spearman test shows a positive correlation between CCL17 and CCL22 with HOMA- IR Index (C, D) and BMI (E, F) (n = 20 control subjects and n = 60 morbidly obese patients).

    Article Snippet: Antigen was unmasked with proteinase K (cat#S3020, Dako, Santa Clara, CA) and blocked with 15% horse serum for 1 h. Samples were incubated with the following primary antibodies overnight at 4°C: mouse anti-human CCL17 (1:50, cat#DY364-05, R&D Systems), mouse anti-human CCL22 (1:50, cat#DY336, R&D Systems), goat anti-human CCR4 (1:100, ab1669; Abcam, Cambridge, UK), rabbit anti-human CD3 (1:100, cat#C7930, Sigma-Aldrich, St. Louis, MO), rabbit polyclonal anti-human CD31 (1:50, cat#ab32457, Abcam) and rat anti-human Mac-3 (1:100, cat#sc19991, Santa Cruz Biotechnology, Dallas, TX).

    Techniques: Clinical Proteomics, Comparison, MANN-WHITNEY, Control

    FIGURE 2 Expression of CCL17 and CCL22 is increased in VCAT from morbidly obese patients. Relative quantification of mRNA levels for (A) CCL17 and (B) CCL22. Comparisons between groups were made by Wilcoxon matched-pair signed-rank test. Values are expressed as mean ± SEM (n = 33). (C) CCL17 and (D) CCL22 chemokine release into conditioned media was determined after 48 h of SCAT and VCAT explant culture. Chemokine secretion is expressed as pg/ml in the supernatant. Values are expressed as mean ± SEM (n = 22). Comparison between groups were made by Mann Whitney test. (E) Immunofluorescence representative images showing colocalization of CCL17 with CD3 (lymphocytes), CD31 (endothelial cells) and Mac-3 (macrophages); or CCL22 with CD3, CD31, Mac-3 in VCAT. Immunoreactivity was visualized using Alexa Fluor 594 (CCL17 and CCL22, red) and Alexa Fluor 488 (CD31, CD3, Mac-3, green) secondary antibodies. Nuclei were stained with Hoechst (blue). Scale bar, 20 mm. Nuclei were stained with Hoechst (blue).

    Journal: Frontiers in endocrinology

    Article Title: CCL17 and CCL22 chemokines are upregulated in human obesity and play a role in vascular dysfunction.

    doi: 10.3389/fendo.2023.1154158

    Figure Lengend Snippet: FIGURE 2 Expression of CCL17 and CCL22 is increased in VCAT from morbidly obese patients. Relative quantification of mRNA levels for (A) CCL17 and (B) CCL22. Comparisons between groups were made by Wilcoxon matched-pair signed-rank test. Values are expressed as mean ± SEM (n = 33). (C) CCL17 and (D) CCL22 chemokine release into conditioned media was determined after 48 h of SCAT and VCAT explant culture. Chemokine secretion is expressed as pg/ml in the supernatant. Values are expressed as mean ± SEM (n = 22). Comparison between groups were made by Mann Whitney test. (E) Immunofluorescence representative images showing colocalization of CCL17 with CD3 (lymphocytes), CD31 (endothelial cells) and Mac-3 (macrophages); or CCL22 with CD3, CD31, Mac-3 in VCAT. Immunoreactivity was visualized using Alexa Fluor 594 (CCL17 and CCL22, red) and Alexa Fluor 488 (CD31, CD3, Mac-3, green) secondary antibodies. Nuclei were stained with Hoechst (blue). Scale bar, 20 mm. Nuclei were stained with Hoechst (blue).

    Article Snippet: Antigen was unmasked with proteinase K (cat#S3020, Dako, Santa Clara, CA) and blocked with 15% horse serum for 1 h. Samples were incubated with the following primary antibodies overnight at 4°C: mouse anti-human CCL17 (1:50, cat#DY364-05, R&D Systems), mouse anti-human CCL22 (1:50, cat#DY336, R&D Systems), goat anti-human CCR4 (1:100, ab1669; Abcam, Cambridge, UK), rabbit anti-human CD3 (1:100, cat#C7930, Sigma-Aldrich, St. Louis, MO), rabbit polyclonal anti-human CD31 (1:50, cat#ab32457, Abcam) and rat anti-human Mac-3 (1:100, cat#sc19991, Santa Cruz Biotechnology, Dallas, TX).

    Techniques: Expressing, Comparison, MANN-WHITNEY, Staining

    (A) Melanoma cells commonly metastasize to the lung, generating surface lesions detectable as pigmented spots. (B) In human melanoma, Treg are detectable by nuclear FoxP3 expression (red) and membrane CD3 staining (blue) resulting in double stained cells (purple arrows) that can be enumerated as a fraction of total T cells (C) Vaccination using plasmid DNA encoding Ccl22 is applied to ventral murine skin to provide proof of concept for (D) diverting Treg away from the circulation and towards skin.

    Journal: Cancer research

    Article Title: CCL22 DIVERTS T REGULATORY CELLS AND CONTROLS THE GROWTH OF MELANOMA

    doi: 10.1158/0008-5472.CAN-16-0618

    Figure Lengend Snippet: (A) Melanoma cells commonly metastasize to the lung, generating surface lesions detectable as pigmented spots. (B) In human melanoma, Treg are detectable by nuclear FoxP3 expression (red) and membrane CD3 staining (blue) resulting in double stained cells (purple arrows) that can be enumerated as a fraction of total T cells (C) Vaccination using plasmid DNA encoding Ccl22 is applied to ventral murine skin to provide proof of concept for (D) diverting Treg away from the circulation and towards skin.

    Article Snippet: Primary antibodies include clones Ta99 to TRP-1 (Covance, Madison, WI), polyclonal rabbit antiserum to mouse and human FoxP3 (ThermoFisher, Rockford, IL) or MF-14 PE rat monoclonal to mouse FoxP3 (Biolegend, San Diego, CA), 145-2C11 bio, FITC or PE to mouse CD3ε (BD Biosciences, Franklin Lakes, NJ) or HIT3A FITC to human CD3 (BD Biosciences), PK138 Alexa Fluor 488 to mouse NK1.1 (BioLegend, San Diego, CA), 8P16-N4-L21 to human CCL1 (Enzo Life Sciences, Farmingdale, NY), 57203 to human CCL22 (R&D Systems, Minneapolis, MN) and MAB4391 to mouse Ccl22 (R&D Systems), TG6/CCR4 to human CCR4 (BioLegend, San Diego, CA) and 191704 to human CCR8 (R&D Systems).

    Techniques: Expressing, Membrane, Staining, Plasmid Preparation

    (A) Expression of melanoma marker TRP-1 in a spinal metastasis is inversely related to (B) FoxP3 expression detected just outside a melanoma lesion whereas (C) such FoxP3 expression colocalizes with CCR4 chemokine receptor expression and (D) faint CCL22 expression detected in the same area. (E) CCR8 chemokine receptor expression and TRP-1 expression are likewise readily mutually exclusive whereas (F) CCL1 expression again colocalizes with its receptor CCR8 in areas surrounding the metastasis. In metastatic lesions of the (G) lung, (H) lymph node and (I) brain, similar distributions of CCL22-expressing cells are observed. Under (J) we quantified CCL22 expression in 3 melanoma in situ samples compared to 4 unaffected adult skin samples, detecting 5-fold upregulated chemokine expression whereas (K) an 8-fold upregulation of CCL1 expression was observed.

    Journal: Cancer research

    Article Title: CCL22 DIVERTS T REGULATORY CELLS AND CONTROLS THE GROWTH OF MELANOMA

    doi: 10.1158/0008-5472.CAN-16-0618

    Figure Lengend Snippet: (A) Expression of melanoma marker TRP-1 in a spinal metastasis is inversely related to (B) FoxP3 expression detected just outside a melanoma lesion whereas (C) such FoxP3 expression colocalizes with CCR4 chemokine receptor expression and (D) faint CCL22 expression detected in the same area. (E) CCR8 chemokine receptor expression and TRP-1 expression are likewise readily mutually exclusive whereas (F) CCL1 expression again colocalizes with its receptor CCR8 in areas surrounding the metastasis. In metastatic lesions of the (G) lung, (H) lymph node and (I) brain, similar distributions of CCL22-expressing cells are observed. Under (J) we quantified CCL22 expression in 3 melanoma in situ samples compared to 4 unaffected adult skin samples, detecting 5-fold upregulated chemokine expression whereas (K) an 8-fold upregulation of CCL1 expression was observed.

    Article Snippet: Primary antibodies include clones Ta99 to TRP-1 (Covance, Madison, WI), polyclonal rabbit antiserum to mouse and human FoxP3 (ThermoFisher, Rockford, IL) or MF-14 PE rat monoclonal to mouse FoxP3 (Biolegend, San Diego, CA), 145-2C11 bio, FITC or PE to mouse CD3ε (BD Biosciences, Franklin Lakes, NJ) or HIT3A FITC to human CD3 (BD Biosciences), PK138 Alexa Fluor 488 to mouse NK1.1 (BioLegend, San Diego, CA), 8P16-N4-L21 to human CCL1 (Enzo Life Sciences, Farmingdale, NY), 57203 to human CCL22 (R&D Systems, Minneapolis, MN) and MAB4391 to mouse Ccl22 (R&D Systems), TG6/CCR4 to human CCR4 (BioLegend, San Diego, CA) and 191704 to human CCR8 (R&D Systems).

    Techniques: Expressing, Marker, In Situ

    To demonstrate that Ccl22 vaccination increases the fraction of skin Treg without converting T cells into Treg, we exposed 3 samples of T cells from a FoxP3 reporter mouse to Ccl22 or to TGF-β overnight. Splenocytes were gated for CD3+ T cells and exposed to CD4 and CD25 immunostaining. (A) FoxP3+CD25+ Treg in a representative untreated sample, (B) Treg abundance is increased in a representative sample of TGFβ treated cells whereas (C) exposure to Ccl22 does not induce a Treg phenotype in vitro. (D) Tregs are quantified, demonstrating a significant increase in response to 2 and 20 ng/mL TGF-β of up to 60%. (E) In a representative transwell migration assay among 3 performed, Treg abundance is increased among splenocytes migrating towards Ccl22, whereas (F) preferential Treg migration is not observed towards Ccl1.

    Journal: Cancer research

    Article Title: CCL22 DIVERTS T REGULATORY CELLS AND CONTROLS THE GROWTH OF MELANOMA

    doi: 10.1158/0008-5472.CAN-16-0618

    Figure Lengend Snippet: To demonstrate that Ccl22 vaccination increases the fraction of skin Treg without converting T cells into Treg, we exposed 3 samples of T cells from a FoxP3 reporter mouse to Ccl22 or to TGF-β overnight. Splenocytes were gated for CD3+ T cells and exposed to CD4 and CD25 immunostaining. (A) FoxP3+CD25+ Treg in a representative untreated sample, (B) Treg abundance is increased in a representative sample of TGFβ treated cells whereas (C) exposure to Ccl22 does not induce a Treg phenotype in vitro. (D) Tregs are quantified, demonstrating a significant increase in response to 2 and 20 ng/mL TGF-β of up to 60%. (E) In a representative transwell migration assay among 3 performed, Treg abundance is increased among splenocytes migrating towards Ccl22, whereas (F) preferential Treg migration is not observed towards Ccl1.

    Article Snippet: Primary antibodies include clones Ta99 to TRP-1 (Covance, Madison, WI), polyclonal rabbit antiserum to mouse and human FoxP3 (ThermoFisher, Rockford, IL) or MF-14 PE rat monoclonal to mouse FoxP3 (Biolegend, San Diego, CA), 145-2C11 bio, FITC or PE to mouse CD3ε (BD Biosciences, Franklin Lakes, NJ) or HIT3A FITC to human CD3 (BD Biosciences), PK138 Alexa Fluor 488 to mouse NK1.1 (BioLegend, San Diego, CA), 8P16-N4-L21 to human CCL1 (Enzo Life Sciences, Farmingdale, NY), 57203 to human CCL22 (R&D Systems, Minneapolis, MN) and MAB4391 to mouse Ccl22 (R&D Systems), TG6/CCR4 to human CCR4 (BioLegend, San Diego, CA) and 191704 to human CCR8 (R&D Systems).

    Techniques: Immunostaining, In Vitro, Transwell Migration Assay, Migration

    (A) In a representative experiment of 2 performed, 5 mice/group were gene gun vaccinated with Ccl22-encoding or empty vector DNA as shown. (B) At euthanasia, lung tissues were collected to identify tumors (C) Significantly reduced pulmonary tumor counts were observed in Ccl22 vaccinated mice as quantified for 5 mice/group (P<0.05). (D) In therapeutic experiments, mice were tumor challenged 3 days before vaccination applied every 5 days as shown (E) Examples of pulmonary tissues from the Ccl22 (n=7) and empty vector control (n=8) groups. (F) Trimmed means (highest and lowest values removed from each group) were used to calculate that significantly reduced portions of the lung surface were covered by tumor in Ccl22 mice (representative experiment of 2 performed).

    Journal: Cancer research

    Article Title: CCL22 DIVERTS T REGULATORY CELLS AND CONTROLS THE GROWTH OF MELANOMA

    doi: 10.1158/0008-5472.CAN-16-0618

    Figure Lengend Snippet: (A) In a representative experiment of 2 performed, 5 mice/group were gene gun vaccinated with Ccl22-encoding or empty vector DNA as shown. (B) At euthanasia, lung tissues were collected to identify tumors (C) Significantly reduced pulmonary tumor counts were observed in Ccl22 vaccinated mice as quantified for 5 mice/group (P<0.05). (D) In therapeutic experiments, mice were tumor challenged 3 days before vaccination applied every 5 days as shown (E) Examples of pulmonary tissues from the Ccl22 (n=7) and empty vector control (n=8) groups. (F) Trimmed means (highest and lowest values removed from each group) were used to calculate that significantly reduced portions of the lung surface were covered by tumor in Ccl22 mice (representative experiment of 2 performed).

    Article Snippet: Primary antibodies include clones Ta99 to TRP-1 (Covance, Madison, WI), polyclonal rabbit antiserum to mouse and human FoxP3 (ThermoFisher, Rockford, IL) or MF-14 PE rat monoclonal to mouse FoxP3 (Biolegend, San Diego, CA), 145-2C11 bio, FITC or PE to mouse CD3ε (BD Biosciences, Franklin Lakes, NJ) or HIT3A FITC to human CD3 (BD Biosciences), PK138 Alexa Fluor 488 to mouse NK1.1 (BioLegend, San Diego, CA), 8P16-N4-L21 to human CCL1 (Enzo Life Sciences, Farmingdale, NY), 57203 to human CCL22 (R&D Systems, Minneapolis, MN) and MAB4391 to mouse Ccl22 (R&D Systems), TG6/CCR4 to human CCR4 (BioLegend, San Diego, CA) and 191704 to human CCR8 (R&D Systems).

    Techniques: Plasmid Preparation

    Treg in formalin-fixed cryosections by immunofluorescent double staining. CD3 shown in green and FoxP3 staining in red. DAPI counterstaining was used. Shown is a sequence representing (A) FoxP3 stained and (B) CD3 stained lung tissue as well as (C) an overlay, in an untreated mouse, including DAPI counterstaining and (D) the number of Treg was quantified as a ratio to total infiltrating T cells in pulmonary tissue, revealing a marked reduction in Ccl22 vaccinated mice (n=3, *P<0.05), representative experiment of 2 evaluated. The overall abundance of pulmonary (E) T cells and (F) NK cells was not affected. (G) Example TUNEL stainings of lung tissue from empty vector (top) and Ccl22 (bottom) treated mice in a prophylactic experiment. (H) The number of Treg is elevated in the skin of vaccinated mice. (I) Tregs in a sample of Ccl22 vaccinated skin.

    Journal: Cancer research

    Article Title: CCL22 DIVERTS T REGULATORY CELLS AND CONTROLS THE GROWTH OF MELANOMA

    doi: 10.1158/0008-5472.CAN-16-0618

    Figure Lengend Snippet: Treg in formalin-fixed cryosections by immunofluorescent double staining. CD3 shown in green and FoxP3 staining in red. DAPI counterstaining was used. Shown is a sequence representing (A) FoxP3 stained and (B) CD3 stained lung tissue as well as (C) an overlay, in an untreated mouse, including DAPI counterstaining and (D) the number of Treg was quantified as a ratio to total infiltrating T cells in pulmonary tissue, revealing a marked reduction in Ccl22 vaccinated mice (n=3, *P<0.05), representative experiment of 2 evaluated. The overall abundance of pulmonary (E) T cells and (F) NK cells was not affected. (G) Example TUNEL stainings of lung tissue from empty vector (top) and Ccl22 (bottom) treated mice in a prophylactic experiment. (H) The number of Treg is elevated in the skin of vaccinated mice. (I) Tregs in a sample of Ccl22 vaccinated skin.

    Article Snippet: Primary antibodies include clones Ta99 to TRP-1 (Covance, Madison, WI), polyclonal rabbit antiserum to mouse and human FoxP3 (ThermoFisher, Rockford, IL) or MF-14 PE rat monoclonal to mouse FoxP3 (Biolegend, San Diego, CA), 145-2C11 bio, FITC or PE to mouse CD3ε (BD Biosciences, Franklin Lakes, NJ) or HIT3A FITC to human CD3 (BD Biosciences), PK138 Alexa Fluor 488 to mouse NK1.1 (BioLegend, San Diego, CA), 8P16-N4-L21 to human CCL1 (Enzo Life Sciences, Farmingdale, NY), 57203 to human CCL22 (R&D Systems, Minneapolis, MN) and MAB4391 to mouse Ccl22 (R&D Systems), TG6/CCR4 to human CCR4 (BioLegend, San Diego, CA) and 191704 to human CCR8 (R&D Systems).

    Techniques: Double Staining, Staining, Sequencing, TUNEL Assay, Plasmid Preparation